XVII-ICSMV

Discovering the potential of biomaterial-based mesoporous and magneto-luminescent nanohybrid (Nd-doped Hydroxyapatite/Fe3O4) for cancer theranosis

Not scheduled

1h 30m

Museo Caracol (Ensenada, México)

Oral Luminescence Phenomena: Materials and Applications LUMINESCENCE PHENOMENA

Speaker

Dr Prakhar Sengar (Centro de Nanociencias y Nanotecnología, Universidad Nacional Autónoma de México Ensenada, Baja California, México, C.P. 22860)

Description

Multimodality nanoplatforms play a crucial role in advancing medical interventions by integrating multiple functionalities into a single system. However, issues like intricate production processes and biocompatibility persist. Herein, a facile synthesis of a biomaterial-based mesoporous nanocarrier, HAp:Nd+SPIONs@mSiO2, loaded with the near-infrared (NIR) emitting dye indocyanine green (ICG) is reported.HAp:Nd nanoparticles were synthesized via combustion methods. Thereafter, commercial SPIONs and HAp:Nd were integrated within a mesoporous silica via a modified Stöber approach. HAp:Nd+SPIONs@mSiO2 nanoplatform was characterized for particle size, porosity, and luminescence using TEM, BET, and luminescence spectroscopy. The synthesized nanoplatform was further loaded with ICG dye and the loading efficiency was analyzed via UV-Vis spectroscopy. Photonic and magnetic thermal heating of the ICG-loaded nanoplatform was also analyzed along with the photo-stimulated ROS generation ability. Finally, cytotoxicity and therapeutic analysis was performed in vitro.The nanohybrid with ~100 nm average size, comprised of Nd-doped hydroxyapatite (HAp), Fe3O4 superparamagnetic iron oxide nanoparticles (SPIONs), and mesoporous silica, exhibiting magneto-luminescent properties. The mesoporous structure was loaded with ICG as a model drug (4.3 μg/mg of nanoparticles) where a pH-dependent release was observed. The nanocarrier demonstrated dual functionality by generating heat through magnetic and photonic stimulation, as well as producing reactive oxygen species (ROS) upon excitation with 808 nm light. In vitro bioevaluation on aggressive triple-negative breast cancer cells (MDA-MB-231) showed the high biocompatibility of nanohybrid with and without ICG and exhibited significant toxicity after irradiation of NIR light. Noticeably, the nanohybrids also exhibit the ability to monitor temperature changes via Nd3+ associated NIR luminescence. The nanoplatform integrates clinically relevant components, highlighting its potential for translation from the laboratory to clinical applications. The developed nanohybrids, with combined NIR-mediated photothermal and photodynamic effects, magnetic photothermal capabilities, and NIR/MR imaging, offer promise in addressing cancer heterogeneity and improving conventional treatments with reduced side effects.

Reference

Corresponding author's email address: prakharsengar@ens.cnyn.unam.mx, hirata@ens.cnyn.unam.mx

P. Sengar, K. Chauhan, and G.A. Hirata, Progress on carbon dots and hydroxyapatite based biocompatible luminescent nanomaterials for cancer theranostics, Transl Oncol 24 (2022) 101482. https://doi.org/10.1016/J.TRANON.2022.101482.

This work was supported by

DGAPA-UNAM (Grant IN-113823 and IA-201124) and the Consejo Nacional de Ciencia y Tecnología CONACyT-México (Grant CB-284548). Dr. Prakhar Sengar gratefully acknowledges CONACyT for the postdoctoral fellowship.